Metoprolol Succinate (MS) is B1 -Blocker acting as a adrenoreceptor antagonist agent and Olmesartan medoxomil (OM) is an AT1- receptor blocking property so, the combination is used in the treatment of Hypertension. MS and OM were estimated at 254nm by densitometry using silica gel 60/UV254 as stationary phase and a premix of Toluene: Methanol: Acetonitrile: Triethylamine (4:2:4:0.2 v/v) as mobile phase. Rf value of MS and OM were 0.39±0.02 and 0.66±0.02 respectively, with a Linearity range 500-3000 ng/band and 200-1200 ng/band and R2 value was found to be 0.997 and 0.999 for MS and OM respectively. The developed high performance thin layer chromatography method was found to be specific, precise and reproducible and can be used for the routine estimation of MS and OM in the combined tablet dosage form, available in market
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